## ShortCut[R]: locator

Welcome to my new category: ShortCut! Here I’ll shortly explain some smart features, unknown extensions or uncommon pathways of going for gold. Today it’s about the Gnu R tool locator.

With locator you are able to detect the mouse position inside you plot. Just run locator() and click some points, when you’re finished click the right button and locator will print the x - and y -values of the clicked positions. With this tool it’s possible to visually validate some numerical calculation.

With a little bit more code, you can output the coordinates right into you plot:

With a click into the plot you’ll be able to create a result like figure 1.

## RNA-Seq - introducing Galaxy

I’m actually attending a lecture with the great name RNA-Seq, dealing with next generation sequencing (NGS). I think the lecture is more or less addressed to biological scientist and people who are working with genome analyzers, but I think there is no harm in visiting this lecture and to get to know the biologists point of view.

These scientists are using different sequencing platforms. Some popular examples are Roche 454, Illumina/Solexa, ABI SOLiD, Pacific Biosciences PacBio RS, Helicos HeliScope™ Single Molecule Sequencer or Polonator, but there are much of more such platforms. If you are interested in these different techniques, you are referred to [Met09]. There is no standard, so all these machines produce output in different formats and quality. In general you’ll get a fastq file as result of sequencing. This file contains roughly more or less small reads of sequences and a quality score of each recognized nucleotide. The quality score is encoded in ASCII characters and contains four line types. Here is an example of such a file:

As you can see, in general the file contains an identifier line, starting with @ , the recognized sequence, a comment, starting with + , followed by the quality score for each base. It’s a big problem that there is no common standard for these quality scores, they differ in domain depending on the sequencing platform. So the original Sanger format uses PHRED scores ([EG98] and [EHWG98]) in an ASCII range 33-126 ( ! - ~ ), Solexa uses Solexa scores encoded in ASCII range 59-126 ( ; - ~ ) and with Illumina 1.3+ they introdused PHRED scores in an ASCII range 64-126 ( @ - ~ ). So you sometimes won’t be able to determine which format your fastq file comes from, the Illumina scores can be observed by all of this three example. If you want to learn more about fastq files and formats you are referred to [CFGHR10]. Interested readers are free to translate the ASCII coded quality scores of my small example to numerical quality scores and post the solution to the comment!

There is a great tool established to work with these resulting fastq files (this is just a small field of application): Galaxy. It is completely open source and written in Python. Those who already worked with it told me that you can easily extend it with plug-ins. You can choose wheter to run your own copy of this tool or to use the web platform of the Penn State. There’s a very huge ensemble of tools, I just worked with a small set of it, but I like it. It seems that you are able to upload unlimited size of data and it will never get deleted!? Not bad guys! You can share your data and working history and you can create workflows to automatize some jobs. Of course I’m excited to write an en- and decoder for other data like videos or music to and from fastq - let’s see if there’s some time ;-)

But this platform also has some inelegance’s. So there is often raw data presented in an raw format. Have a look at figure 1, you can see there is a table, columns are separated by tabs, but if one word in a column is much smaller/shorter as another one in this column this table looses the human readability! Here I’ve colorized the columns, but if the background is completely white, you have no chance to read it.

So instead of getting angry I immediately wrote a user-script. It adds a button on the top of pages with raw data and if it is clicked, it creates an HTML table of this data. You can see a resulting table in figure 2. If you think it is nice, just download it at the end of this article.

All in all I just can estimate what’s coming next!

# References

[CFGHR10]
Peter J. A. Cock, Christopher J. Fields, Naohisa Goto, Michael L. Heuer, and Peter M. Rice. The Sanger FASTQ file format for sequences with quality scores, and the Solexa/Illumina FASTQ variants. Nucleic Acids Research, 38(6):1767–1771, April 2010. http://nar.oxfordjournals.org/content/38/6/1767.abstract
[EG98]
Brent Ewing and Phil Green. Base-Calling of Automated Sequencer Traces Using Phred. II. Error Probabilities. Genome Research, 8(3):186–194, March 1998. http://www.ncbi.nlm.nih.gov/pubmed/9521922
[EHWG98]
Brent Ewing, LaDeana Hillier, Michael C. Wendl, and Phil Green. Base-Calling of Automated Sequencer Traces Using Phred. I. Accuracy Assessment. Genome Research, 8(3):175–185, March 1998. http://www.ncbi.nlm.nih.gov/pubmed/9521921
[Met09]
Michael L. Metzker. Sequencing technologies — the next generation. Nature Reviews Genetics, 11(1):31–46, December 2009. http://www.nature.com/nrg/journal/v11/n1/full/nrg2626.html

## Resort search results in SquirrelMail

Apart from an IMAP/POP service we provide a webmail front end to interact with our mail server via SquirrelMail. This tool has a very annoying feature, search results are ordered by date, but in the wrong way: From old to new!

SquirrelMail is a very simple to administrate front end, not very nice, but if my experimental icedove doesn’t work I use it too. Furthermore we have staff members, who only use this tool and aren’t impressed by real user-side clients like icedove or sylpheed.. What ever, I had to resort these search results!

Searching for a solutions doesn’t result in a solution, so I had three options: Modifying the SquirrelMail code itself (very bad idea, I know), providing a plugin for SquirrelMail, or writing a userscript.

Ok, hacking the core of SquirrelMail is deprecated, writing a plugin is to much work for now, so I scripted some JavaScript.

The layout of this website is lousy! I think the never heard of div’s or CSS, everything is managed by tables in tables of tables and inline layout specifications -.- So detecting of the right table wasn’t that easy. I had to find the table that contains a headline with the key From :

If I’ve found such a table, all the rows have to be sorted from last to first. Except the first ones defining the headline of that table. So I modified the DOM:

Ok, that’s it! Using this script the search results are ordered in the correct way. Let’s wait for a response from these nerdy SquirrelMail-user ;-)

## Welcome Rumpel!

Ladies and gentlemen, waiting is finally over. I’m proud to introduce Rumpel!

After more than one year of high frequently power of persuasion he finally set up his own blog named RforRocks. A few minutes after release there are even lots of myths surrounding the origin of that name. May it stand for:

Who knows? Me not! So try to grill Rumpel about this issue. And by the way, he’s in any case worthy to follow ;-) (so Rumpel, time for my payoff)

## Reminded by unforgotten

Who doesn’t know, yesterday was your wife’s birthday and you didn’t bought some flowers yet!? Sounds like trouble, but these days are gone!

Some days ago I read an article at chruetertee.ch about the BSD tool calendar. I immediately realized, that this tool is the answer to one of my biggest questions: How to remember all the dates!?

Today I found some time to implement a small bash script that sends mails based on the output of the calendar tool. So it helps you remembering this periodical stuff.

On the software page for this script you can learn how to use it. It is more than simple ;-)

Now I need to collect all important dates, so if YOU want me to wish you all the best at your birthday you have to leave a comment or write me an email!